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KMID : 0364819870250030244
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Korean Journal of Microbiology
1987 Volume.25 No. 3 p.244 ~ p.248
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Differentiation of mixed bacterial populations by modified Gram stain
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Abstract
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1
Attempts were made to enumerate the number of Gram positive and negative bacteria in the development of natural fermentation rapidly and simultaneously. A general Gram stain was applied to this study. The number of cells by Gram stain was proportional to the cell turbidity by spectrophotometer within a range of 0.7 absorbance at 610nm. The cells washed out during procedures were not exceeded about 8 percentage. The standard error of separate counts in the mixture of
Escherichia coil and Micrococcus luteus was 5.1 ± 2.3%. The possible range of counting was 5.5 x 107-1.0 x 10´cel *, W. Therefore, it is believed that a general Gram stain could be applied to the
separate counting of mixture of Gram positive and negative bacterial populations too. In practice, growth kinetics of hemp retting and Kimchi fermentation were presented.
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